ISOLATION OF COATED VESICLES, PLAIN SYNAPTIC VESICLES, AND FLOCCULENT MATERIAL FROM A CRUDE SYNAPTOSOME FRACTION OF GUINEA PIG WHOLE BRAIN
نویسندگان
چکیده
منابع مشابه
Isolation of Coated Vesicles, Plain Synaptic Vesicles, and Flocculent Material from a Crude Synaptosome Fraction of Guinea Pig Whole Brain
Two vesicular fractions and one nonvesicular fraction were prepared from crude synaptosomes by differential centrifugation and salting out with ammonium sulfate. Fraction 1 contained a mixture of coated vesicles, material thought to be derived from breakdown of the coats (shell fragments), and plain synaptic vesicles. Fraction 2 contained a mixture of plain synaptic vesicles and flocculent mate...
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Brief internalization of [125I]transferrin was used to label coated endocytic vesicles, which were then purified using a combination of 2H20 and 2H20/ Ficoll density gradients. Purification was monitored using an assay measuring fusion of endocytic organelles, so as to isolate functional vesicles. Isolated vesicles had all the properties of clathrin-coated vesicles, being enriched for the major...
متن کاملIsolation of Functional, Coated, Endocytic Vesicles
Brief internalization of [125I]transferrin was used to label coated endocytic vesicles, which were then purified using a combination of 2H20 and 2H20/ Ficoll density gradients. Purification was monitored using an assay measuring fusion of endocytic organelles, so as to isolate functional vesicles. Isolated vesicles had all the properties of clathrin-coated vesicles, being enriched for the major...
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Brief internalization of [125I]transferrin was used to label coated endocytic vesicles, which were then purified using a combination of 2H20 and 2H20/ Ficoll density gradients. Purification was monitored using an assay measuring fusion of endocytic organelles, so as to isolate functional vesicles. Isolated vesicles had all the properties of clathrin-coated vesicles, being enriched for the major...
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Brief internalization of [125I]transferrin was used to label coated endocytic vesicles, which were then purified using a combination of 2H2O and 2H2O/Ficoll density gradients. Purification was monitored using an assay measuring fusion of endocytic organelles, so as to isolate functional vesicles. Isolated vesicles had all the properties of clathrin-coated vesicles, being enriched for the major ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1973
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.58.1.135